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. 2008 Nov;10(6):520–526. doi: 10.2353/jmoldx.2008.080024

Figure 4.

Figure 4

Assay sensitivity for detection of KRAS point mutations at codon 12 in homogeneous and mixed cell populations. A: Sensitivity of the PNA clamp SMAP-2 assay on a homogeneous target. PNA clamp SMAP-2 assay with serial dilutions of SW480 cell line genomic DNA. Shown are 1000 copies (closed circles), 500 copies (closed squares), 100 copies (open circles), 50 copies (open squares), 10 copies (open triangles), and 0 copy (x). Ten copies of mutant DNA are detected in a 26-μl reaction in ∼45 minutes. B: Sensitivity of the PNA clamp SMAP-2 assay on a heterogeneous target. PNA clamp SMAP-2 assay detects mutations in a mixture of wild-type genomic DNA. Shown are 50% (closed circles), 10% (closed squares), 1% (open circles), 0.5% (open squares), 0.1% (open triangles), and 0% (x) of SW480 cell line genomic DNA. Even 0.1% of mutant DNA can be detected in a 26-μl reaction in ∼50 minutes.