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. 2008 Oct 1;9:56. doi: 10.1186/1471-2172-9-56

Figure 3.

Figure 3

Flow cytometry of expanded cells before and after culturing for a subsequent week in G-CSF. Expression of CD16 and CD66 was up-regulated in HPC expanded in IL-3 and IL-6 stimulated EC cultures (p < 0.05), while in IL-1β cultures, no further up-regulation was observed. Increase of granulocytic glycoproteins occurred in parallel to the development of granulocytic morphology. Pictures were taken from one representative result of six independent experiments. A) forward scatter – side scatter, IgG control; B) CD16 and CD66 expression before culturing with G-CSF; C) CD16 and CD66 expression and cell morphology after culturing with G-CSF.