Table 1.

Cell expansion in IL-stimulated endothelial supernatant following a period of 7 and 14 days and flow cytometric profile on day 7.

	Concentration (U/ml)	Fold increases		Flow cytometry
		7 days	14 days	CD33, 34, 45, 14, 16, 133
After isolation		N/A	N/A
Control	Non-stimulated	1.2 ± 0.13	6.4 ± 1.2
	0.1% BSA	1.3 ± 0.17	6.8 ± 1.6
IL-1β	1	5 ± 1a	6.3 ± 0.12	N/A
	10	13 ± 2.2a,b	59.1 ± 13.3a,b
	100	15.8 ± 2.5a,b	136.8 ± 23.3a,b
	1,000	19.4 ± 8.6a,b	142.7 ± 23.5a,b
	10,000	10.5 ± 2.8a,b	97 ± 22.4a,b
IL-3	10	11.8 ± 1.6a,b	71.2 ± 10.5a,b
	100	15.5 ± 1.6a,b	82.9 ± 14.5a,b
	1,000	15.2 ± 1.4a,b	79.6 ± 9.9a,b
IL-6	10	1.5 ± 0.51	2.8 ± 0.58
	100	1.2 ± 0.22	5.2 ± 1.2
	1,000	1.3 ± 0.21	4.6 ± 0.77

Fold expansions were determined following a period of seven and fourteen days. Percentage of CD33, 34, 45, 14, 16 and CD133 positivity are depicted as circles (○: negative, less than 10%; quarter circle: 10 – 25% positivity; half circle: 25 – 50% positivity; ● more than 75% positive cells).

a: highly significant different compared to non-stimulated supernatant (p < 0.001); b: highly significant different compared to BSA supernatant (p < 0.001). Shown are mean results ± SE of twelve independent experiments. N/A: not applicable.