Table 3.
Cobblestone area and long-term culture initiating cells (LTC-IC) of HPC post-isolation and of cells cultured in IL-stimulated EC supernatant for one week.
| Concentration (U/ml) | CAFC | LTC-IC | ||
| 2-week | 5-week | |||
| Post isolation | N/A | 4.9 ± 0.73 | 23.2 ± 4.2 | 16.3 ± 3 |
| Control | No addition | 11.6 ± 2.5c | 16.8 ± 4.5 | 17 ± 4.3 |
| 0.1% BSA | 9.6 ± 2.8 | 17.8 ± 4.5 | 16.2 ± 4.2 | |
| IL-1β | 10 | 3.7 ± 1.1 | 5.4 ± 2.4a,c | 7 ± 2.2 |
| 100 | 4.9 ± 0.95 | 3.4 ± 1.2a,b,c | 6.6 ± 2.3 | |
| 1,000 | 5.6 ± 0.92 | 0.45 ± 0.2a,c | 3.7 ± 2.2c | |
| 10,000 | 19.2 ± 7.1b,c | 0.38 ± 0.17a,b,c | n.d. | |
| IL-3 | 10 | 3.6 ± 1.1a,c | 8.1 ± 2a,c | 3.2 ± 2c |
| 100 | 4.3 ± 1.5a | 2.8 ± 0.58a,b,c | 2.3 ± 0.59c | |
| 1,000 | 2.2 ± 0.5a,c | 4 ± 0.88a,b,c | 1.8 ± 0.61c | |
| IL-6 | 10 | 3.6 ± 1.1 | 9 ± 2 | 11 ± 3.2 |
| 100 | 4.3 ± 1.5 | 20.9 ± 4.1 | 13.2 ± 6.3 | |
| 1,000 | 2.2 ± 0.5a,b | 15.7 ± 3.3 | 8.5 ± 3.4 | |
Freshly isolated and expanded HPC were cultured on the murine bone marrow stroma cell line MS-5 and scored for cobblestone-area formation after two and five weeks. LTC-IC were scored by replating 5-week CAFC in methylcellulose for secondary colony formation. Shown are mean results ± SD of three independent experiments in triplicate; a: significant different compared to non-stimulated supernatant (p < 0.05); b: significant different compared to BSA supernatant (p < 0.05); c: significant different compared to freshly isolated CD34(+) cells.