Table 4.

Burst activities of differentiated cells expanded in IL-stimulated endothelial supernatant.

	Control	E. coli	fMLP	PMA
IL-1β (FBS)	52.5 ± 13.1 (593 ± 156.9)	509.5 ± 107.9 (169.1 ± 30.6)	62.7 ± 10.5 (1336 ± 320.5)	350.4 ± 95.5 (2873.4 ± 615.9)
IL-3	55.4 ± 13.2	800.7 ± 343.1	74 ± 33.1	784.3 ± 334.7
IL-6	39 ± 9.4	467.6 ± 167.31	177 ± 124	287.1 ± 104.5

After culturing the cells for one week with G-CSF (100 ng/ml) and keeping them overnight in human serum, expanded cells showed a ten-fold increased burst activity in response to E. coli and PMA. Oxygen radical formation in cells from different interleukin conditions were comparable (p > 0.05). Shown are average results of mean fluorescence activities ± SE from five independent experiments. In brackets: mean results after storage in FBS-based medium (n = 10).

PMA: phorbol 12-myristate 13-acetate; E. coli: Escherichia coli; fMLP: N-formyl-methionyl-leucyl-phenylalanin.