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Suppression of PUMA expression abrogated the apoptosis induced by H2O2. LoVo PUMA wild-type cells and LoVo PUMA_AS cells were treated with H2O2 (0.64 mM) for 15 minutes and allowed to recover for 24 hours. Hoechst dye 33258 was used to detect apoptosis. Data are presented as mean number of apoptotic cells (mean ± SD) from three independent experiments. Significant difference between LoVo PUMA wild-type cells and LoVo PUMA_AS cells is indicated by * (p < 0.05).
