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Native chemical ligation of RNase A-MESNA to Cys-PEG-DSPE. a) Structure formula of Cys-PEG-DSPE lipids. b) SDS-PAGE analysis of the reaction of refolded RNase A-MESNA (lane 1) (~10 μM) in HBS pH 7.4 with 50 mM MPAA and 1 mM Cys-PEG-DSPE (lane 2), or in HBS pH 7.4 with 50 mM MPAA, 10 mM TCEP and 1 mM Cys-PEG-DSPE (lane 3) at 20°C for 20 h.
