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. 1992 May;60(5):1756–1760. doi: 10.1128/iai.60.5.1756-1760.1992

Effects of endotoxin-associated protein on hematopoiesis.

R Porat 1, M Yanoov 1, M A Johns 1, S Shibolet 1, R Michalevicz 1
PMCID: PMC257069  PMID: 1563761

Abstract

Endotoxin-associated protein (EAP), a gram-negative bacterial cell wall component, was evaluated for its effects on hematopoietic colony formation in vitro. Colony-stimulating activity, induced by EAP on circulating and bone marrow progenitor cells, was found to be partially mediated by T cells and augmented by interleukin-3. The addition of anti-human interleukin-1 (IL-1) antibodies reduced EAP activity, suggesting that EAP may induce IL-1 production. However, EAP was shown to promote the growth of mature progenitor cells independently, unlike the effects of IL-1 on the hematopoietic system. These studies demonstrate that bacterial components other than lipopolysaccharide, such as EAP, may have hematopoietic activity.

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Selected References

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  1. Bagby G. C., Jr, Dinarello C. A., Wallace P., Wagner C., Hefeneider S., McCall E. Interleukin 1 stimulates granulocyte macrophage colony-stimulating activity release by vascular endothelial cells. J Clin Invest. 1986 Nov;78(5):1316–1323. doi: 10.1172/JCI112717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bjornson B. H., Agura E., Harvey J. M., Johns M., Andrews R. G., McCabe W. R. Endotoxin-associated protein: a potent stimulus for human granulocytopoietic activity which may be accessory cell independent. Infect Immun. 1988 Jun;56(6):1602–1607. doi: 10.1128/iai.56.6.1602-1607.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cannon J. G., Tompkins R. G., Gelfand J. A., Michie H. R., Stanford G. G., van der Meer J. W., Endres S., Lonnemann G., Corsetti J., Chernow B. Circulating interleukin-1 and tumor necrosis factor in septic shock and experimental endotoxin fever. J Infect Dis. 1990 Jan;161(1):79–84. doi: 10.1093/infdis/161.1.79. [DOI] [PubMed] [Google Scholar]
  4. Carswell E. A., Old L. J., Kassel R. L., Green S., Fiore N., Williamson B. An endotoxin-induced serum factor that causes necrosis of tumors. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3666–3670. doi: 10.1073/pnas.72.9.3666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dinarello C. A., Cannon J. G., Wolff S. M., Bernheim H. A., Beutler B., Cerami A., Figari I. S., Palladino M. A., Jr, O'Connor J. V. Tumor necrosis factor (cachectin) is an endogenous pyrogen and induces production of interleukin 1. J Exp Med. 1986 Jun 1;163(6):1433–1450. doi: 10.1084/jem.163.6.1433. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dinarello C. A. Interleukin-1 and interleukin-1 antagonism. Blood. 1991 Apr 15;77(8):1627–1652. [PubMed] [Google Scholar]
  7. Doe W. F., Yang S. T., Morrison D. C., Betz S. J., Henson P. M. Macrophage stimulation by bacterial lipopolysaccharides. II. Evidence for differentiation signals delivered by lipid A and by a protein rich fraction of lipopolysaccharides. J Exp Med. 1978 Aug 1;148(2):557–568. doi: 10.1084/jem.148.2.557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Goodman G. W., Sultzer B. M. Endotoxin protein is a mitogen and polyclonal activator of human B lymphocytes. J Exp Med. 1979 Mar 1;149(3):713–723. doi: 10.1084/jem.149.3.713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hogan M. M., Vogel S. N. Lipid A-associated proteins provide an alternate "second signal" in the activation of recombinant interferon-gamma-primed, C3H/HeJ macrophages to a fully tumoricidal state. J Immunol. 1987 Dec 1;139(11):3697–3702. [PubMed] [Google Scholar]
  10. Iscove N. N., Shaw A. R., Keller G. Net increase of pluripotential hematopoietic precursors in suspension culture in response to IL-1 and IL-3. J Immunol. 1989 Apr 1;142(7):2332–2337. [PubMed] [Google Scholar]
  11. Johns M. A., Sipe J. D., Melton L. B., Strom T. B., McCabe W. R. Endotoxin-associated protein: interleukin-1-like activity on serum amyloid A synthesis and T-lymphocyte activation. Infect Immun. 1988 Jun;56(6):1593–1601. doi: 10.1128/iai.56.6.1593-1601.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kimball H. R., Melmon M. L., Wolff S. M. Endotoxin-induced kinin production in man. Proc Soc Exp Biol Med. 1972 Mar;139(3):1078–1082. doi: 10.3181/00379727-139-36302. [DOI] [PubMed] [Google Scholar]
  13. Leary A. G., Ikebuchi K., Hirai Y., Wong G. G., Yang Y. C., Clark S. C., Ogawa M. Synergism between interleukin-6 and interleukin-3 in supporting proliferation of human hematopoietic stem cells: comparison with interleukin-1 alpha. Blood. 1988 Jun;71(6):1759–1763. [PubMed] [Google Scholar]
  14. Michalevicz R., Katz F., Stroobant P., Janossy G., Tindle R. W., Hoffbrand A. V. Platelet-derived growth factor stimulates growth of highly enriched multipotent haemopoietic progenitors. Br J Haematol. 1986 Jul;63(3):591–598. doi: 10.1111/j.1365-2141.1986.tb07537.x. [DOI] [PubMed] [Google Scholar]
  15. Michalevicz R., Lifshitz D., Revel M. Interferon beta 2/interleukin-6 and interleukin-3 synergize in stimulating proliferation of human early hematopoietic progenitor cells. Scanning Microsc. 1989 Dec;3(4):1143–1150. [PubMed] [Google Scholar]
  16. Michie H. R., Manogue K. R., Spriggs D. R., Revhaug A., O'Dwyer S., Dinarello C. A., Cerami A., Wolff S. M., Wilmore D. W. Detection of circulating tumor necrosis factor after endotoxin administration. N Engl J Med. 1988 Jun 9;318(23):1481–1486. doi: 10.1056/NEJM198806093182301. [DOI] [PubMed] [Google Scholar]
  17. Moore M. A., Warren D. J. Synergy of interleukin 1 and granulocyte colony-stimulating factor: in vivo stimulation of stem-cell recovery and hematopoietic regeneration following 5-fluorouracil treatment of mice. Proc Natl Acad Sci U S A. 1987 Oct;84(20):7134–7138. doi: 10.1073/pnas.84.20.7134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Morrison D. C., Betz S. J. Chemical and biological properties of a protein-rich fraction of bacterial lipopolysaccharides. II. The in vitro rat peritoneal mast cell response. J Immunol. 1977 Nov;119(5):1790–1795. [PubMed] [Google Scholar]
  19. Morrison D. C., Ryan J. L. Bacterial endotoxins and host immune responses. Adv Immunol. 1979;28:293–450. doi: 10.1016/s0065-2776(08)60802-0. [DOI] [PubMed] [Google Scholar]
  20. Morrison D. C., Ulevitch R. J. The effects of bacterial endotoxins on host mediation systems. A review. Am J Pathol. 1978 Nov;93(2):526–618. [PMC free article] [PubMed] [Google Scholar]
  21. Mortensen R. F., Shapiro J., Lin B. F., Douches S., Neta R. Interaction of recombinant IL-1 and recombinant tumor necrosis factor in the induction of mouse acute phase proteins. J Immunol. 1988 Apr 1;140(7):2260–2266. [PubMed] [Google Scholar]
  22. Staber F. G., Habild W. F., Morrison D. C., Tarcsay L. Hemopoietic effects in mice of a lipid A-associated protein. Exp Hematol. 1981 Mar;9(3):264–273. [PubMed] [Google Scholar]
  23. Sultzer B. M., Goodman G. W. Endotoxin protein: a B-cell mitogen and polyclonal activator of C3H/HeJ lymphocytes. J Exp Med. 1976 Sep 1;144(3):821–827. doi: 10.1084/jem.144.3.821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Tewari A., Buhles W. C., Jr, Starnes H. F., Jr Preliminary report: effects of interleukin-1 on platelet counts. Lancet. 1990 Sep 22;336(8717):712–714. doi: 10.1016/0140-6736(90)92206-w. [DOI] [PubMed] [Google Scholar]
  25. Vogel S. N., Kaufman E. N., Tate M. D., Neta R. Recombinant interleukin-1 alpha and recombinant tumor necrosis factor alpha synergize in vivo to induce early endotoxin tolerance and associated hematopoietic changes. Infect Immun. 1988 Oct;56(10):2650–2657. doi: 10.1128/iai.56.10.2650-2657.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. WOLFF S. M., RUBENSTEIN M., MULHOLLAND J. H., ALLING D. W. COMPARISON OF HEMATOLOGIC AND FEBRILE RESPONSE TO ENDOTOXIN IN MAN. Blood. 1965 Aug;26:190–201. [PubMed] [Google Scholar]
  27. Wankowicz Z., Megyeri P., Issekutz A. Synergy between tumour necrosis factor alpha and interleukin-1 in the induction of polymorphonuclear leukocyte migration during inflammation. J Leukoc Biol. 1988 Apr;43(4):349–356. doi: 10.1002/jlb.43.4.349. [DOI] [PubMed] [Google Scholar]
  28. Warren D. J., Moore M. A. Synergism among interleukin 1, interleukin 3, and interleukin 5 in the production of eosinophils from primitive hemopoietic stem cells. J Immunol. 1988 Jan 1;140(1):94–99. [PubMed] [Google Scholar]
  29. Zucali J. R., Dinarello C. A., Oblon D. J., Gross M. A., Anderson L., Weiner R. S. Interleukin 1 stimulates fibroblasts to produce granulocyte-macrophage colony-stimulating activity and prostaglandin E2. J Clin Invest. 1986 Jun;77(6):1857–1863. doi: 10.1172/JCI112512. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Zuckerman S. H., Bendele A. M. Regulation of serum tumor necrosis factor in glucocorticoid-sensitive and -resistant rodent endotoxin shock models. Infect Immun. 1989 Oct;57(10):3009–3013. doi: 10.1128/iai.57.10.3009-3013.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]

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