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Western blot of Asb3 in C57BL/6J, DBA/2J, BXD25, and BXD40. C57BL/6J (B6) and BXD25 show elevated Asb3 protein abundance relative to DBA/2J (D2) and BXD40. The uniform intensity of the β-tubulin staining across all samples validates the consistency of the total protein loaded for each strain.
