Figure 3.

Activity of wild-type and FKBP12 mutants in the peptide-based prolyl isomerase assays. (A) Results for the wild-type protein (circles) and the F106Y mutant (squares). (B) Results for the F43Y (inverted triangles) and the D44V mutants (triangles). Activities measured by the protease-coupled assay are shown by the open symbols; activities measured by the protease-free peptide assays are shown by the solid symbols. The activities are given as k/k₀, where k and k₀ are the rate constants of isomerization in the presence and absence of these proteins, respectively. The activities were determined at 15°C as described in MATERIALS AND METHODS.