Figure 6.

Expression of heterologous FKBP domains in yeast FKBP12 mutants. The 5′ and 3′ regulatory regions of the yeast FKBP12 gene were fused to the open reading frames from human FKBP12, yeast FKBP13 lacking the secretory signal sequence, and yeast-human and human-yeast chimeric FKBP12 genes. The resulting genes were expressed from a CEN plasmid in vph6 fpr1 and fpr1 mutant strains CHY516 and JHY2–1c, as in Figure 1, to assay complementation and restoration of drug sensitivity. S, R/S, and R indicate sensitivity, partial sensitivity, and resistance, respectively. Complementation of the growth defect of FKBP12 mutant strain JHY2–1c by heterologous FKBP domains was assessed on solid medium and by determining doubling times of the transformants grown in liquid culture (YPD medium) at 30°C. +, complementation; −, no complementation.