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In vitro folding activities of wild-type and mutant FKBP12
| FKBP | Protease-coupled assay (kcat/Km[M−1·s−1]) | Protease-free assay (kcat/Km[M−1·s−1]) | Protein folding assay (kcat/Km[M−1·s−1]) |
|---|---|---|---|
| Wild-type | 740,000 | 810,000 | 49,000 |
| F106Y | 85,000 | 111,000 | 29,000 |
| F43Y | <2,000 | 34,000 | 13,000 |
| D44V | <3,000 | 46,000 | 51,000 |
