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Confocal images of PDHC E1 α and NeuN immunoreactivity in the hippocampal CA3 region of a sham-operated (S) compared with a hyperoxic resuscitated animal (H). Robust PDHC immunostaining apparent in the cell bodies of pyramidal neurons in the nonischemic animal is lost in the hyperoxic resuscitated animal. Loss of PDHC immunoreactivity is not due to loss of neurons as NeuN immunostaining remains relatively unchanged. Scale bar = 50 μ m.
