FIG. 1.

Anti-IL-23 antibody is specific for mouse IL-23 and neutralizes IL-23 bioactivity. (A) Mouse IL-12, IL-23, or p40 homodimer were coated on ELISA plates and anti-IL-23 antibody was titrated. Data are shown as the mean optical density (OD) of replicate wells ± SD and are representative of two experiments. IL-17 or IFN-γ production was measured in supernatants from C57BL/6 splenocyte cultures supplemented with recombinant mouse (B) IL-23 or (C) IL-12 and dilutions of anti-IL-12/23p40, anti-IL-12p35, or anti-IL-23p19 antibodies. Data are shown as the mean of replicate wells ± SEM and are representative of three experiments.