FIG. 4.

Blocking IL-12/23 does not alter T-cell infiltration into the CNS of MHV-infected mice. MHV-infected mice were treated with either anti-IL-23p19 or anti-12/23p40 neutralizing antibodies and the effects on T-cell infiltration into the CNS evaluated at specific time points post-infection. Blocking either IL-23 (A) or IL-12/23 (B) did not reduce total numbers of CD4⁺ or CD8⁺ T cells entering the CNS when compared to mice treated with control antibody. Similarly, treatment with anti-IL-23p19 (C) or anti-IL-12/23p40 (D) did not alter infiltration of virus-specific CD4⁺ or CD8⁺ T cells compared to control-antibody-treated mice, as shown in representative FACS dot-plots and corresponding bar graphs. The bar graphs portray the frequency of virus-specific CD4⁺ or CD8⁺ T cells within their respective T-cell subset on days 7 and 14 post-infection. The frequencies of virus-specific T cells were derived from dual-positive IFN-γ⁺ CD4⁺/CD8⁺ populations as shown in boxed regions of representative dot-plots from day 7 post-infection. Data are presented as average ± SEM and are representative of two independent experiments with a total n = 4–8 for each antibody treatment group per time point examined.