Figure 5.

Expression and Numbers of Cyclotides within a Plant Species.

LC-MS profiles of the plant extracts from O. affinis (A), A. robijnsii (B), and C. discolor (C) and are shown to highlight the number of unique cyclotides in each plant species. LC elution profiles are shown from 10 to 70 min at 1% min⁻¹ solvent B (solvent B: 90% acetonitrile in Milli-Q H₂O with 0.1% formic acid). Elutions of cyclotides, characterized by mass spectrometry, are indicated with Arabic numbers. Cyclotide masses are given to the right of the graph. Some cyclotides coelute from the reverse phase C18 column; hence, multiple masses are indicated. The representative plant species screened from O. affinis contains at least 18, A. robijnsii at least 22, and C. discolor at least 38 unique and novel cyclotides.