Figure 1.

Expression of the MPC Gene.

(A) Allele-specific sequencing of MPC and control cDNAs from Col × Ler and Ler × Col siliques at 5 DAP. Only the maternal alleles of MPC and FWA are represented in the sequence for both crosses (polymorphic nucleotide in black box). The nonimprinted control gene, αVPE, shows a double peak at the polymorphic nucleotide.

(B) RT-PCR and restriction analysis of cDNA from seeds dissected into embryo and endosperm+seed coat fractions at 7 DAP. MPC is expressed only from maternal alleles in endosperm+seed coat, but biallelic in embryo. The imprinted FWA gene is a positive control for the dissection, and αVPE is a nonimprinted control.

(C) RT-PCR analysis of MPC cDNA from vegetative and reproductive organs. MPC is primarily expressed in flower buds, female reproductive organs of open flowers, and young siliques. GAPC, control; WP, whole plant; R, root 5 weeks old; RL, rosette leaf; CL, cauline leaf; S, stem; SL, seedling; BL, late floral buds; OF, open flowers; OF-P, open flowers without pistils; MS, mature stamens; S3, siliques at 3 DAP; S5, siliques 5 DAP; S7, siliques 7 DAP; SE3, seed 3 DAP; SW3, silique wall 3 DAP; SE5, seed 5 DAP; SW5, silique wall 5 DAP; Em7, embryo dissected from 7 DAP seed; En7, endosperm+seed coat dissected from 7 DAP seed.