Figure 6.

MPC Is Methylated in the 5′ Flanking Region and in the Gene Body.

(A) RT-PCR of MPC and surrounding regions treated with the methylation-sensitive restriction endonuclease McrBC (+) or mock treated (–). McrBC digests methylated DNA; the presence of a PCR product indicates a lack of methylation in that genomic fragment. Genomic DNA was extracted from rosette leaf of met1-6 or wild-type Col plants. The start codon is at position +1, and the positions of other gene features are indicated in the schematic below.

(B) CG methylation in multiple clones of the upstream region and gene body of MPC determined by bisulfite sequencing of rosette leaf DNA in met1-9 and wild-type Col backgrounds. A black circle indicates a methylated cytosine in CG context, an open circle is an unmethylated cytosine, and a gray circle is a methylated cytosine in a CN context where N is an unknown base. There are no CG sites between +25 and +45.