Figure 1.

Endogenous SLR1 Protein Levels Correlate with the Severity of Dwarfism in GA-Deficient and GA-Insensitive Mutants, with the Exception of gid2.

(A) Gross morphology of GA-deficient mutants (left panel) and various alleles of gid1 and gid2 mutants (right panel) grown for 2 weeks. The wild type is shown as a control. Closed and open arrowheads represent the uppermost positions of the 2nd and 3rd leaf sheaths, respectively. Bars = 5 cm.

(B) Top panel: SLR1 protein in the plant lines from (A) detected by protein gel blot analysis. SLR1-P, phosphorylated SLR1; SLR1, nonphosphorylated SLR1. Bottom panel: Coomassie blue (CBB) staining to show that approximately equal amounts of total protein (10 μg) were loaded in each lane.