Figure 2.

Effects of GA on Dwarfism and Levels of SLR1 Protein in the Wild Type, gid2, and gid1.

(A) Gross morphology of the wild type, gid2-2, and gid1-3 under GA-rich or GA-deficient conditions. The seedlings were grown with (uni +, GA₃ −) or without (uni −, GA₃ −) 10⁻⁶ M uniconazole (an inhibitor of GA synthesis) for 3 weeks, or the seedlings were grown with 10⁻⁶ M uniconazole for 2 weeks and then treated with GA₃ for 1 more week (uni +, GA₃ +). Closed arrowheads represent the uppermost positions of the 4th leaf sheath. Bar = 5 cm.

(B) Top panel: protein gel blot showing the level of SLR1 protein in seedlings grown under the conditions presented in (A). Bottom panel: Coomassie blue (CBB) staining to show approximately equal amounts of total protein (10 μg) were loaded in each lane.

(C) Time course of GA-dependent accumulation of SLR1 in gid2-2. The seedlings were pretreated with 10⁻⁶ M uniconazole for 2 weeks and then treated with GA₃ for the indicated period.