Figure 6.

Effect of the ga1-3 Mutation on sly1-10 Growth and RGA Protein Accumulation.

(A) The 21-d-old seedlings of ga1-3, sly1-10, and the ga1-3 sly1-10 double mutant in the absence (−GA) and presence (+GA) of 100 μM GA₄. Bar = 5 mm.

(B) The effect of GA treatment (100 μM GA₃ treatment) on accumulation of RGA protein in the ga1-3, sly1-10, and ga1-3 sly1-10 mutants. Plants (35 d old) were treated with GA, and time points were taken as indicated for protein blot analysis. sly1-10 is a control for equal loading. Protein was extracted from rosette leaves.

(C) RGA mRNA accumulation in rosette leaves of the ga1-3, sly1-10, and ga1-3 sly1-10 mutants was determined at time points indicated after GA treatment by quantitative RT-PCR. Mean values for at least three independent experiments are shown. Error bars show sd.