Figure 5.

The C-Terminal IMS Region of ARC6 Is Required for Chloroplast Division Activity but Not for ARC6 Localization.

(A) Schemes showing ARC6-GFP and ARC6_ΔIMS-GFP. The numbers shown beneath the diagrams indicate amino acid positions.

(B) Anti-GFP immunoblot showing relative GFP fusion protein levels in plants shown in (D) and (E).

(C) to (E) Phenotypes of an untransformed arc6 mutant (C), an arc6 mutant expressing ARC6_pro-ARC6-GFP (D), and arc6 mesophyll cells expressing ARC6_pro-ARC6_ΔIMS-GFP (E). Bars = 10 μM.

(F) GFP localization in arc6 mutants expressing 35S_pro-ARC6_ΔIMS-GFP. Arrowheads indicate ring localization of ARC6_ΔIMS-GFP at sites of constriction within the chloroplasts of young leaf cells. Bar = 10 μM.