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. 2008 Nov 4;2(11):e328. doi: 10.1371/journal.pntd.0000328

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Serial 2-fold dilutions of M. leprae were made from 2×10⁶ to 1.56×10⁴/ml. Ten microliters of each dilution were tested in triplicate representing 2×10⁴ to 156 M. leprae in the test sample, respectively. The ordinate is PCR cycle number at threshold and the abscissa is number of M. leprae (log₁₀). Standard deviations did not exceed 0.5% of mean at any dilution.