Table 2. The number and percent of bacilli recovered from conventional mouse foot pads within 4 hours and 1 week post inoculation with varying doses of M. leprae as measured by RLEP PCR.
4 hr | % of Dose Retained | 1 wk | % of Dose Retained | |||
Dose Bacilli Given | Site | Bacilli/Foot Pad Recovered | Site | Bacilli/Foot Pad Recovered | ||
1.00E+07 | LF1 | 2.08E+06 | 20.80% | LF4 | 1.25E+05 | 1.25% |
RF1 | 1.48E+06 | 14.80% | RF4 | 2.70E+05 | 2.70% | |
LF2 | 1.15E+06 | 11.50% | LF5 | 3.20E+05 | 3.20% | |
RF2 | 8.67E+05 | 8.67% | RF5 | 7.15E+05 | 7.15% | |
LF3 | 5.61E+05 | 5.61% | ||||
RT3 | 1.74E+06 | 17.40% | ||||
1.00E+06 | LF1 | 1.23E+05 | 12.28% | LF4 | 6.14E+04 | 6.14% |
RF1 | 8.27E+04 | 8.27% | RF4 | 4.95E+04 | 4.95% | |
LF2 | 5.07E+04 | 5.10% | LF5 | 6.68E+03 | 0.67% | |
RF2 | 8.39E+04 | 8.39% | RF5 | 7.80E+04 | 7.80% | |
LF3 | 2.94E+04 | 2.94% | ||||
RF3 | 3.73E+04 | 3.73% | ||||
1.00E+05 | LF1 | NR | NR | LF4 | 2.13E+03 | 2.10% |
RF1 | NR | NR | RF4 | 2.81E+03 | 2.80% | |
LF2 | 6.65E+03 | 6.65% | LF5 | 4.31E+03 | 4.31% | |
RF2 | 2.87E+03 | 2.87% | RF5 | 7.39E+03 | 7.39% | |
LF3 | 2.84E+03 | 2.84% | ||||
RF3 | 2.86E+03 | 2.86% | ||||
Average (SD) | 8.42% (5.57%) | 4.21% (2.47%) |
LF = Left Foot with dose animal number, RF = Right Foot with dose animal number, NR = Not Run. SD = Standard Deviation of average percent bacilli retained in the foot pad.