Figure 1.

Identification of desaturase in liver microsomes from rats that have undergone dietary alteration. (A) SDS-PAGE, Coomassie blue-stained gel. Lanes 1 and 7 denote molecular weight standards with the mass marked on the left of the panel. Lane 2, pyrophosphate-washed and lane 5, high-salt–washed microsomes from desaturase induced animals. Lane 3 corresponds to microsomes from control animals; lane 4, purified desaturase; lane 6, postmicrosomal supernatant from desaturase-induced liver. (B) Immunoblot analysis of desaturase in the above fractions. Samples of identical amounts to those shown in Figure 1A were electrophoresed on SDS-PAGE, transferred onto polyvinylidene difluoride Immobilon-P membrane, and incubated with antibody against desaturase, followed by incubation with secondary antibody (anti-rabbit IgG-alkaline phosphatase). The bands were visualized using phosphatase substrate detection kit as described in MATERIALS AND METHODS.