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Association of desaturase with the components of subcellular fractions. Lane 2, P-1 (800 × g); lane 3, P-2 (10,000 × g) fractions. Lanes 5 and 6, Triton X-114 extract of P-2 fraction: lane 5, detergent-rich phase; lane 6, detergent-depleted phase. (A) Coomassie Blue-stained gel. (B) The above gel immunoblotted with antidesaturase antibody as in Figure 1B.
