Figure 6.

Microsomal degradation of desaturase is not inhibited by pepstatin or leupeptin. Experimental conditions were as in Figure 4 except that pepstatin (240 μM) was present in reaction mixtures represented by lanes 4 and 5 and leupeptin (400 μM) by lanes 6 and 7. (A) Coomassie Blue-stained gel. (B) Immunoblot of the above gel with antidesaturase antibody as in Figure 1B.