Figure 4.

The response of the retina and RPE to light damage is modified by constant light preconditioning. A, RNA blots showing Serpin a3n and Osmr transcript accumulation in BALB/c mouse retina either preconditioned for 5 d in constant light (CL; ∼100 lux) or maintained in darkness (DA; dark adapted) and then exposed for 1.5 h to 6000 lux with pupil dilation, followed by a 24 h recovery in darkness. B, Immunoblot showing OSMR accumulation in the retina and RPE of BALB/c mice treated as described in A. C, In situ hybridization to Serpin a3n, Cebpd, and Mmp3 transcripts in the RPE and/or retina 24 h after light damage to BALB/c mice that were either dark adapted or constant light preconditioned. Light-damage induction of RPE transcripts is suppressed by preconditioning. D, In situ hybridization to Serpin a3n and Osmr transcripts in constant light-preconditioned mice after retinal detachment. Induction of these transcripts in retina and RPE is not suppressed by preconditioning. Vertical bars to the right of each tissue section are described in Figure 1.