FIGURE 4.

Expression of LRP and up-regulation of α₂-macroglobulin in glaucoma. Low magnification pictures of intact retina and glaucomatous retina immunostained with antibodies LRP-1 (A and B) and α2M (F and G). RGCs were first retrogradely labeled with fluorogold, intact or glaucoma retinas were dissected, and were immunostained with antibodies to LRP-1 (C–E) α2M (H and J). Retinas were dissected from glaucomatous eyes (high IOP day 28) and sections were immunostained with antibodies to glial fibrillary acidic protein (GFAP) and LRP-1 (K) or α2M (L). Data were acquired by fluorescence microscopy. RPE, retinal pigment epithelium; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bars, 50 A, B, F, G, K, and L) and 25 μm (C–E and H–J).