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Dose-dependent decrease in GAG biosynthesis by 4-deoxy-4-fluoroxylosides in CHO cells. Samples of 3H-labeled GAG chains from control and 4-deoxy-4-fluoroxyloside-treated (compounds 5e (A) and 5g (B)) CHO cells were applied to a DEAE HPLC column, eluted with a linear NaCl gradient starting with 0.2 m NaCl as described under “Experimental Procedures,” and analyzed for radioactivity with an in-line radiodetector.
