FIGURE 3.

HAT1 active site mutants are defective in telomeric silencing. A, point mutants of hat1 harboring the indicated histone H3 alleles were assayed for telomeric silencing. Telomeric silencing was measured by spotting 10-fold serial dilutions of cells on synthetic complete plates (SC) and synthetic complete plates containing 5-FOA. Plates were photographed after 3 days of growth at 30 °C. The relevant genotypes of the strains are indicated on the left. Assays were performed on at least three independent isolates from each strain. B, Western blot analyses were performed on normalized total protein samples to verify equivalent protein expression utilizing peroxidase-anti-peroxidase antibody. WT, wild type.