FIGURE 4.

A, effect of pH on TGP1 activity. The maximum activity obtained at pH 3.4 was taken as 100% relative activity. B, effect of temperature on TGP1 stability and activity. For stability experiments (broken line), TGP1 was preincubated at various temperatures for 30 min, cooled to 4 °C and assayed at 30 °C. Stability is measured relative to samples stored on ice. For temperature optimum experiments (solid line), end point fluorescence was monitored after 15 min of incubation of enzyme and substrate at defined temperatures. Activity was calculated relative to maximum fluorescence at 50 °C.