TABLE 1.

Purification of extracellular TGP1 and PEP1 from T. emersonii

Sample	Purification step	Total volume	Total protein	Total activity	Specific activity	Yield	Purification factor
		ml	mg	units	units/mg	%	-fold
Crude	Spent media	12,100	1077	28333	26.31	100	1
	Concentrate/dialyzed	20	820	26445	32.25	93.34	1.23
	DEAE-cellulose	90	380	25145	66.17	88.75	2.52
TGP1	DEAE-cellulose	60	51.5	16459	319.59	58.09	12.15
	Sephacryl S-100	33	19.83	15000	756.43	52.94	28.75
PEP1	DEAE-cellulose	70	20.5	3625	176.83	12.79	6.72
	Sephacryl S-100	40	5.3	1684	317.74	5.94	12.07
	TEAE-cellulose	12	0.35	245.2	700.57	0.87	26.6