FIGURE 10.

P473L protein forms elevated levels of ATP-independent cleavage complexes in the presence of mAMSA. ATP-dependent (A) and ATP-independent (B) cleavage complex formation was assessed using a gel cleavage assay in the presence of 5 μg/ml mAMSA. The amount of added protein is indicated in micrograms above each lane. The lane labeled Sc contains supercoiled pUC18, and the lane labeled L contains pUC18 DNA linearized by EcoRI. WT, wild-type. The mobilities of nicked (N), linear (L), and supercoiled (Sc) are indicated to the right of each gel. Note that in this experiment, unlike those shown in Figs. 8 and 9, the same mass of each enzyme was added, as indicated.