FIGURE 6.

Stimulatory role of iNOS in human platelet aggregation and secretion. A and B, representative aggregation and secretion traces of washed human platelets preincubated for 5 min at 37 °C with 100 μm iNOS-specific inhibitors 1400W or aminoguanidine (AG) and stimulated with 0.02 unit/ml thrombin (A) or 0.7 μg/ml collagen (B). The statistical difference between control and inhibitor-treated platelets was analyzed using a paired t test with following results: A, 1400W aggregation p = 0.0024, n = 4, 1400W secretion p = 0.0442, n = 4; aminoguanidine aggregation p = 0.0018, n = 3, aminoguanidine secretion p = 0.0227, n = 3; B, 1400W aggregation p = 0.0025, n = 4, 1400W secretion p = 0.0374, n = 4; aminoguanidine aggregation p = 0.0138, n = 4, aminoguanidine secretion p = 0.0136, n = 3.