Role of PKG in sildenafil (SIL)-induced expression of Bcl-2 and
Bax. A, isolated mouse hearts infused with sildenafil (1
μm) for 10 min in the absence or presence of KT5823 (1 mg/kg).
B, mouse cardiomyocytes treated with sildenafil (1 μm)
in the absence or presence of KT5823 (2 μm) for 1 h. C,
densitometry analysis of the Bcl-2/actin ratio (* indicates
p < 0.05 versus control and KT + sildenafil; n =
3); D, Bax/actin ratio (n = 3; NS); E, Bcl-2/Bax
ratio (* indicates p < 0.05 versus control and
KT + sildenafil; ** indicates p < 0.01 versus
control and KT + sildenafil; n = 3).