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Effect of ERK inhibitor, PD98059, on anti-necrotic and anti-apoptotic effects of SIL in mouse cardiomyocytes. Bar diagram showing quantitative data of necrosis (A) and apoptosis (B) (* indicates p < 0.05 versus DMSO + SI-RO control; n = 4). PD98059 was dissolved in DMSO and myocytes were treated with DMSO (as vehicle control) or PD98059 (20 μm) for 1 h prior to 40 min of SI and 1 h of RO (for necrosis) and 18 h of RO (for apoptosis). SIL, sildenafil.
