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. 1997 Nov;8(11):2291–2306. doi: 10.1091/mbc.8.11.2291

Figure 5.

Figure 5

srv2–14, but not srv2Δ, mutations confer an endocytic defect. (A) Endocytosis of radiolabeled pheromone in SRV2 (RH978) and srv2–14 (RH3993) strains was measured at 24°C (solid symbols) or 37°C (open symbols) after a 15-min (SRV2, srv2–14) preincubation at the indicated temperature. Endocytosis in srv2Δ (RH4008, triangles) and SRV2 (RH3393, circles) was measured after a 5-min preincubation at 24°C. (B) Accumulation of LY in SRV2 (RH978) and srv2–14 (RH3993) mutants grown at 24°C and preincubated for 15 min at 37°C prior to addition of LY for 1 h. (C) Maturation of radiolabeled CPY was measured in SRV2 (RH978) and srv2–14 (RH3993) mutants grown at 24°C and preincubated for 15 min at 37°C prior to starting a pulse–chase experiment as described in MATERIALS AND METHODS. p1, endoplasmic reticulum form of CPY; p2, Golgi-modified form of CPY; m, mature vacuolar form of CPY.