Table. Results of different methods used to confirm dengue virus type 4 (DENV-4) infection, Manaus, Amazonas, Brazil*.
| Isolate | IgM antibody capture ELISA | Virus culture† |
M-PCR |
S-PCR |
BLAST | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| D1 | D2 | D3 | D4 | D1 | D2 | D3 | D4 | D1 | D2 | D3 | D4 | |||||
| AM750 | – | – | – | + | + | – | – | + | + | – | – | + | + | DENV-3/ DENV-4‡ | ||
| AM1041 | + | – | – | – | + | – | – | – | + | – | – | – | + | NS | ||
| AM1619 | + | – | – | – | + | – | – | – | + | – | – | – | + | DENV-4 | ||
*IgM, immunoglobulin M; M-PCR, multiplex typing PCR described by Lanciotti et al. (10); S-PCR, single serotype-specific primer PCR; D, dengue serotype; BLAST, basic local alignment search tool; NS, not sequenced. †Viral antigens detected by immunofluorescence with type-specific monoclonal antibodies. ‡Isolate AM750 is from a person with a co-infection; different clones were sequenced.