Table 1. Primers sequences and amplification parameters used in the present study.

Locus	Primer	5′ labeling	Sequence (5′ → 3′)*	Annealing temperature, °C
14αDM†	DMC1		>TGGGTGGTCAACATACTTC	50
	DMC2‡		<CATCTRTGTCTACCACCACC
Actin	ACTa		>AAGGTATTATGGTTGGTATGG	55
	ACTb		<TCGAAATCTAAAGCAACGTAA
URA3	URAF	HEX§	>ATTGGATAGTCCCTCTAAACTCACTACTA	55
	URAr		<AGCATTAGTTATATCACTCCACGATGAA
	URAF2		>TGCCGATATTGGAAATACAGTTA	50
	URAr2		<AATCAACTATTCAAGTTGACCG
	CTU2		<GTTGGAACATCAATTGATGCACATAAAT	55
Unknown	CT14a	6FAM¶	>GTAAATCTTGTATACCGTGGA	55
	CT14b		<TAGCCCATTTTCTAGTTTTGC
FCY1	CTCDf		>ATCATTAGTTCAGATGGTAAAGTCTTG	58
	CTCDr		<CCTTTTTAGTAACATGTCTATTCTCCA
FCY2	CTCP1f		>TGCCCATAAATTAAATGCAGAA	58
	CTCP1r		<GGAAGCAACAAACCCAAAAA
FCY2	CTCP2f		>TGCTGCCGATTATGTTGTTT	58
	CTCP2r		<GTGAAAACGAGCCAATCCAT
FUR1	FUR1f		>TCATCAAAACCATGTCTGCTG	58
	FUR1r		<AAGTGTATGTAGTGATAATTGCTATGC

*>, Sense primer; <, antisense primer.
†14 α demethylase.
‡R = G or A.
§4, 7, 2′,4′, 5′,7′-hexachloro-6-carboxyfluorescein.
¶6-carboxyfluorescein.