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. 2008 Oct 31;3(10):e3542. doi: 10.1371/journal.pone.0003542

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Biswas et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Immuno-staining of forager brain sections (a) for synapsin using SynOrf-1 antibody and incubation with Alexa-488-conjugated anti-mouse antibody allowing green colouration to highlight protein expression; (b) for honeybee neurexin-I using DmNrx-1 antibody and incubation with Alexa-546-conjugated anti-mouse antibody allowing red colouration to highlight protein expression; (c) merge shows neurexin-I and synapsin co-localise in mushroom body neuropil (MB medial lobe and MB calyx), optic lobe neuropil (medulla and lobulla) and antennal lobes. Small puncta of neurexin I expression, distinct to synapsin, are highlighted by small arrow heads within the optic lobe (OL) stratum.