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. 2008 Oct 9;105(42):16101–16106. doi: 10.1073/pnas.0802647105

Fig. 1.

Fig. 1.

Single-molecule force measurement between importin-β and Nup153-C. (A) A schematic illustration of full-length mouse importin-β (residues 1–876). HEAT repeats involved in the pocket formation are indicated. (B) A schematic representation of the single-molecule force measurement system with AFM. The purified Nup153-C was immobilized on a glass substrate. GST-fused importin-β was noncovalently attached to a glutathione-coupled AFM cantilever. The interaction between importin-β and Nup153-C was characterized by monitoring the deflection of the cantilever when the cantilever approaches and then retracts from the glass surface. (C) The histogram of the rupture force obtained in the force measurement. The histogram was fitted with Gaussian distribution. The total number of approaches (N), the number of approaches in which the specific interaction was detected (n) and the mean values of the peaks (f) are also shown.