Fig. 3.

The effect of RanGTP on the FG-binding pockets of importin-β. The rupture force between Nup153-C and importin-β was measured by AFM in the presence (A, B, and E) or absence (D) of RanGTP, and the results were summarized in the histograms. The importin-β constructs used were Imp β-N (A), full-length importin-β (B), Imp βΔIaIb (D and E). The histograms were fitted with Gaussian distribution. N, n, and f are indicated as in Fig. 1C. A schematic illustration of Imp βΔIaIb is shown in C.