Fig. 1.

Cdk1 substrates exhibit differential temporal dephosphorylation. (A) Cell cycle-regulated phosphorylation and dephosphorylation of Pol12 and Sld2. Cells with indicated genotypes were synchronized in G₁ phase with α-factor and then released into YPD medium at 30°C. Cells were collected every 20 min to prepare protein samples. The protein phosphorylation was determined after Western blotting with anti-myc antibody on the basis of band shift. (B) The dephosphorylation of Sld2 and Pol12 depends on Cdc14. G₁-synchronized cells with indicated genotype were released into YPD at 37°C. The phosphorylation of Sld2 and Pol12 was determined as described in A.