Fig. 5.

Nicotine increases GAD₆₇ expression in FC but not in striatum. Mice were treated with nicotine four times a day during the 12 h of light cycle for 4 days. GAD₆₇ was measured 2 h after the last nicotine injection. Each value is the mean ± SE of 5–8 mice. **, P < 0.02; *, P < 0.05 Student's t test between vehicle (VEH) and nicotine treatment. (Inset) Typical Western immunoblot of GAD₆₇ and β-actin after 4–12% SDS/PAGE. Comparison between FC vehicle (lanes 1–3, serial dilutions of the same sample) and nicotine-treated (lanes 4–6, serial dilutions of the same sample) mice.