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. 2008 Oct 13;105(42):16119–16124. doi: 10.1073/pnas.0805240105

Fig. 4.

Fig. 4.

Inhibitor GRL0617 is selective for SARS-CoV PLpro. (A) IC50 values of compounds 5, 6, and GRL0617 are listed for PLpro and 5 other papain-like proteases. (B) Stereo representation of HAUSP (Protein Data Bank ID no. 1NBF, with ubiquitin ligand removed) superimposed with the PLpro-inhibitor complex. HAUSP is shown in green, PLpro in blue, and the inhibitor molecule in orange. HAUSP active site residues that clash with the inhibitor are labeled. (C) An α-HA Western blot of lysed Vero E6 cells treated with HA-Ub-VS in the presence of N-ethyl-maleimide (NEM) or varying concentrations of GRL0617. (D) An α-HA Western blot of lysed Vero E6 cells mixed with 0.1 μg of PLpro and HA-Ub-VS, both in the absence and presence of 40 μM GRL0617.