as2–5D causes ectopic expression of AS2. The expression pattern of pAS2:GUS (A and D), pAS2–5D:GUS (B and E), and pAS2m:GUS (C and F) in Columbia. (A–C) Embryos. (D–F) Eight-day-old seedlings with cotyledons removed to show leaves 1 and 2. The sequence of the region surrounding the as2–5D mutation in each construct is shown, with the mutated nucleotide shown in red. The as2–5D mutation (B and E) causes AS2 to be expressed in the abaxial domains of cotyledons and leaves and in the hypocotyl. (Scale bars: A–C, 20 μm; D–F, 100 μm.)