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. 2008 Oct 13;105(42):16230–16235. doi: 10.1073/pnas.0808830105

Fig. 2.

Fig. 2.

miR-320 directs transcriptional silencing of POLR3D. (A) Mature miR-320 and POLR3D mRNA levels in HEK-293 cells transfected with control or miR-320 duplexes, as measured by TaqMan miRNA assays and qRT-PCR, respectively, and normalized to GAPDH levels (relative expression for miR-320 is indicated in hundreds). (B) Mature miR-21 and miR-29b miRNA levels in HEK-293 cells transfected with control or miR-320 duplexes, as measured by TaqMan miRNA assays and normalized to GAPDH levels. (C) Nuclear run-on experiments for nascent POLR3D mRNA transcription in HEK-293 cells transfected with control or miR-320 duplexes, as measured by qRT-PCR and normalized to nascent GAPDH mRNA transcription levels. (D) ChIP assays, using antibodies to AGO1, EZH2, or H3K27me3 or no antibody (NA) controls, in HEK-293 cells transfected with control or miR-320 duplexes. Quantification of immunoprecipitated APRT (control) and POLR3D promoter regions determined by real-time PCR and normalized to input DNA. Error bars indicate SD (n = 3).