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Persistence of PCNA ubiquitination. PH-XPA cells were irradiated with 20 J m−2 UV, incubated for 6 h, and then photoreactivated for 2 h. After further incubation for the indicated times, PCNA in cell lysates was detected by immunoblotting. In A, duplicate samples were analyzed either with or without prior extraction with Triton X-100, as indicated. (B) Lysates were analyzed for both USP1 and PCNA ubiquitination.
