Figure 1.

FTICR mass spectra of folded and reduced synthetic hepcidin-25. We show the 3⁺ion of unlabelled folded hepcidin 25 (a), reduced unlabelled hepcidin (b), folded labelled hepcidin (c) and reduced labelled hepcidin (d). Spectra were acquired by direct infusion in 0.1% formic acid/50% acetonitrile into a ThermoFinnigan LTQ-FT. Reduced samples were treated with DTT, zip-tipped and kept at low pH prior to analysis.